ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (4): 693-699.doi: 10.11843/j.issn.0366-6964.2016.04.008

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Effects of Lipopolysaccharide on Proliferation,Apoptosis and Estradiol Secretion of in vitro Cultured Porcine Granulosa Cells

GUO Shuang-shuang1,LI Hui2,SHI Zhen-dan2,MA Wei-ming1*   

  1. (1.College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Tai’an 271018,China;2.Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
  • Received:2015-09-25 Online:2016-04-23 Published:2016-04-23

Abstract:

 In order to study the effects of Lipopolysaccharide(LPS) on proliferation,apoptosis and estradiol (E2) secretion of porcine granulosa cells.Primary porcine granulosa cells were cultured in vitro and treated by LPS at different concentrations (0,500,1 000 and 2 000 ng•mL-1).Then cell proliferation,apoptosis,secretion of E2 and related gene expressions,FN1,IGF2,IGFBP2,Cyclin D1,Cyclin D2,P27kip and P450arom were determined individually.The results showed that cell proliferation and survival ratio were significantly promoted at LPS concentrations of 1 000 or 2 000 ng•mL-1(P<0.05),on the contrary,cell apoptosis was inhibited.However,gene expression levels of FN1 (P<0.01),IGF2,IGFBP2 (P<0.05),Cyclin D1 and Cyclin D2 (P<0.01) were up-regulated at 500 ng•mL-1 of LPS,P27kip (P<0.01) which hindered cell cycle was inhibited.P450arom gene expression was decreased,resulting the inhibition of E2 secretion when treated by LPS.These results suggested that granulosa cell proliferation could be promoted by LPS,while cell apoptosis and E2 secretion were inhibited.

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